![]() This technique reduces the non-specific primer binding by decreasing the annealing temperature between two consecutive cycles of PCR. This quantitative real time PCR is used in conjugation with QPCR. This process uses fluorescent dyes to monitor and measure real-time amounts of amplified products. Quantitative Real-Time PCR (QRT-PCR or RTQ-PCR) Q-PCR is also used for the detection of specific sequences inside the DNA sample. Q-PCR is generally used to determine the quantity of PCR product quickly. This is the indirect procedure of measuring initial amounts of RNA, cDNA or template DNA. Further amplification is achieved by carrying out standard PCR in the presence of excess primers for the chosen strand. It is used in the hybridization probing in which only one strand is considered ideal. It selectively amplifies one strand of the template DNA more than the other. (Different types of PCR): RT-PCR uses Fluorophores to detect the levels of gene expression Asymmetric PCR The process includes a sequence of digestion and self-ligation of the template DNA, which results in chunks of DNA with known sequences at the ends of an unknown sequence. This technique is proper in the identification of the flanking sequences to the various gene inserts. It is performed when only single internal sequence of template DNA is known. This technique is exceptionally successful, although it requires much more knowledge of the involved sequences. The subsequent set is expected to amplify the secondary target inside the product of the primary run. Nested PCR is performed with two different sets of primers in two consecutive PCR cycles. This technique is proposed to decrease the contaminants in amplified DNA because of random primer binding amplification. Linear-After-The-Exponential PCR (LATE-PCR).Polymerase Cycling Assembly (PCA) or Assembly PCR.Quantitative Real-Time PCR (QRT-PCR or RTQ-PCR).Polymerase chain reaction (PCR) is broadly classified on the basis of slight modifications in the standard PCR process. ![]() Solved Conceptual MCQs on Different types of PCR. ![]()
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